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Selenophosphate Synthetase 1 antibody

Anti-Selenophosphate Synthetase 1 antibody used in Western Blot (WB). GTX48860

Cat No. GTX48860

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
50 μl ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:100
ELISA 1:5000 - 1:20000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

selenophosphate synthetase 1 , 1110046B24Rik , AA589574 , AI505014 , AW111620 , SELD , SPS , SPS1

Cellular Localization

Cell membrane

Background

Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli. In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis. The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD.

Database

Research Area

DATA IMAGES

Anti-Selenophosphate Synthetase 1 antibody used in Western Blot (WB). GTX48860

GTX48860 WB Image

Western blot using GeneTex's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible cross-reactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf