GeneTex
United States (US)

Selenophosphate synthetase 1 antibody [N1C1-2]

GTX107611_IFA_19011506_647.jpg
GTX107611_39911_IHC_19011506_701.jpg
GTX107611_WB_19011506_365.jpg
GTX107611_39911_20150402_IHC_M_2_19011506_704.jpg
GTX107611_39911_20150402_IHC_M_19011506_513.jpg

Cat No. GTX107611

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Selenophosphate synthetase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Selenophosphate Synthetase 1,Seld,Sps,Sps1,Sephs1

Background

This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107611_IFA_19011506_647.jpg

GTX107611 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Selenophosphate synthetase 1(GTX107611) antibody at 1:200 dilution.

GTX107611_39911_IHC_19011506_701.jpg

GTX107611 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Selenophosphate synthetase 1(GTX107611) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107611_WB_19011506_365.jpg

GTX107611 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX107611 diluted at 1:500

GTX107611_39911_20150402_IHC_M_2_19011506_704.jpg

GTX107611 IHC-P Image

Selenophosphate synthetase 1 antibody [N1C1-2] detects Selenophosphate synthetase 1 protein at cytoplasm and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Selenophosphate synthetase 1 antibody [N1C1-2] (GTX107611) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107611_39911_20150402_IHC_M_19011506_513.jpg

GTX107611 IHC-P Image

Selenophosphate synthetase 1 antibody [N1C1-2] detects Selenophosphate synthetase 1 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Selenophosphate synthetase 1 antibody [N1C1-2] (GTX107611) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169