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Semaphorin 3A antibody

Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550
Anti-Semaphorin 3A antibody used in Western Blot (WB). GTX37550
Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550
Anti-Semaphorin 3A antibody used in IHC (Frozen sections) (IHC-Fr). GTX37550
Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550

Cat. No. GTX37550

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($459)
See all Semaphorin 3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-800
IHC-P 1: 100-500
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human SEMA3A. Please contact us for the exact immunogen sequence.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11176603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

semaphorin 3A , COLL1 , HH16 , Hsema-I , Hsema-III , SEMA1 , SEMAD , SEMAIII , SEMAL , SemD , coll-1

Cellular Localization

Secreted

Background

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550

GTX37550 IHC-P Image

IHC-P analysis of rat liver tissue using GTX37550 Semaphorin 3A antibody.
Dilution : 1:100

Anti-Semaphorin 3A antibody used in Western Blot (WB). GTX37550

GTX37550 WB Image

WB analysis of rat lung (Lane 1), rat brain (Lane 2), mouse brain (Lane 3),rat kidney (Lane 4), rat skin (Lane 5), human breast cancer (Lane 6) tissue lysates using GTX37550 Semaphorin 3A antibody.
Dilution : 0.5μg/ml

Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550

GTX37550 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX37550 Semaphorin 3A antibody.
Dilution : 2.5μg/ml

Anti-Semaphorin 3A antibody used in IHC (Frozen sections) (IHC-Fr). GTX37550

GTX37550 IHC-Fr Image

IHC-Fr analysis of mouse lung tissue using GTX37550 Semaphorin 3A antibody.
Dilution : 1:200

Anti-Semaphorin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX37550

GTX37550 IHC-P Image

IHC-P analysis of rat liver tissue using GTX37550 Semaphorin 3A antibody.
Dilution : 1:200

REFERENCE

Application Reference

Hu R et al. PeerJ 2022; 10 : e13856 Integrated bioinformatics analysis identifies the effects of Sema3A/NRP1 signaling in oligodendrocytes after spinal cord injury in rats.
Sabag AD et al. Immunol Res 2019; (Epub) Altered expression of regulatory molecules in the skin of psoriasis.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459