APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
4-6μg/ml |
Note :
IHC-P
Human Kidney shows membanous and vesicular staining in PCT cells.
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Not tested in other applications.
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89289-PEP).
Product Note
The immunizing peptide corresponds to a part of the extracellular domain.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
semaphorin 5A , SEMAF , semF
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
Database
Research Area
DATA IMAGES
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GTX89289 IHC-P Image
IHC-P analysis of human kidney using GTX89289 Semaphorin 5A antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 4μg/ml
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REFERENCE
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REVIEW
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