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Semaphorin 5A antibody [N1N2], N-term

Anti-Semaphorin 5A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117160
Anti-Semaphorin 5A antibody [N1N2], N-term used in Western Blot (WB). GTX117160

Cat. No. GTX117160

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Semaphorin 5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Zebrafish, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Semaphorin 5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172285

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

semaphorin 5A , SEMAF , semF

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development (Adams et al., 1996 [PubMed 8817451]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Semaphorin 5A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117160

GTX117160 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Semaphorin 5A(GTX117160) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Semaphorin 5A antibody [N1N2], N-term used in Western Blot (WB). GTX117160

GTX117160 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX117160 diluted at 1:2000

REFERENCE

Application Reference

Guoqing Pan et al. Mol Ther Oncolytics 2021; 22 : 256-264 Helicobacter pylori promotes gastric cancer progression by upregulating semaphorin 5A expression via ERK/MMP9 signaling.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169