GeneTex
United States (US)

Separase antibody [XJ11-1B12]

GTX16170_1091_WB_18121410_834.jpg

Cat No. GTX16170

Host Mouse
Clonality Monoclonal
Clone Name XJ11-1B12
Isotype IgG1
Application WB
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
Not tested in other applications.

Calculated MW

233 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Extra Spindle Pole Bodies Like 1, Separase,Esp1,Sepa,Espl1

Cellular Localization

Cytoplasm,Nucleus

Background

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX16170_1091_WB_18121410_834.jpg

GTX16170 WB Image

WB analysis of 5C6 cell lysate using GTX16170 Separase antibody [XJ11-1B12].
Lane 1: non-induced lysate
Lane 2 : lysate induced with ponasterone

Package List Price ($)
$ 329