GeneTex
United States (US)

Separase antibody [XJ11-4D7]

GTX15955_137_ICC-IF_18121410_293.jpg

Cat No. GTX15955

Host Mouse
Clonality Monoclonal
Clone Name XJ11-4D7
Isotype IgG2a
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

233 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Maltose-Binding Protein fusion of C-terminal fragment of human Separase (amino acids 1866-1996). [UniProt# Q14674]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Extra Spindle Pole Bodies Like 1, Separase,Esp1,Sepa,Espl1

Cellular Localization

Cytoplasm,Nucleus

Background

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX15955_137_ICC-IF_18121410_293.jpg

GTX15955 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15955 Separase antibody [XJ11-4D7].
Red : primary antibody
Green : gamma tubulin
Blue : DAPI

Package List Price ($)
$ 319