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Septin 2 antibody [N1N3]

Anti-Septin 2 antibody [N1N3] used in Western Blot (WB). GTX101693
Anti-Septin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101693
Anti-Septin 2 antibody [N1N3] used in Western Blot (WB). GTX101693
Anti-Septin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101693

Cat. No. GTX101693

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Septin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Septin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241305

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

septin 2 , DIFF6 , NEDD-5 , NEDD5 , Pnutl3 , hNedd5

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Database

Research Area

DATA IMAGES

Anti-Septin 2 antibody [N1N3] used in Western Blot (WB). GTX101693

GTX101693 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody [N1N3] (GTX101693) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Septin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101693

GTX101693 IHC-P Image

Septin 2 antibody [N1N3] detects Septin 2 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Septin 2 antibody [N1N3] (GTX101693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Septin 2 antibody [N1N3] used in Western Blot (WB). GTX101693

GTX101693 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX101693 diluted at 1:2000
Anti-Septin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101693

GTX101693 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using SEPT2 (GTX101693) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169