Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody [N1N3] (GTX101693) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using SEPT2 (GTX101693) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX101693 diluted at 1:2000

Septin 2 antibody [N1N3] detects Septin 2 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Septin 2 antibody [N1N3] (GTX101693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min