GeneTex
United States (US)

Septin 2 antibody [N1N3]

GTX101693_39596_IHC_M_19011506_727.jpg
GTX101693_IHC_19011506_638.jpg
GTX101693_WB_19011506_536.jpg

Cat No. GTX101693

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , Jurkat , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Septin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIFF6 antibody, KIAA0158 antibody, NEDD5 antibody, Pnutl3 antibody, hNedd5 antibody, SEPT2 antibody, NEDD-5 antibody, neural precursor cell expressed developmentally down-regulated protein 5 antibody, septin-2 antibody, "neural precursor cell expressed, developmentally down-regulated 5 antibody", septin 2 antibody

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Database

Research Area

DATA IMAGES
GTX101693_39596_IHC_M_19011506_727.jpg

GTX101693 IHC-P Image

Septin 2 antibody [N1N3] detects Septin 2 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Septin 2 antibody [N1N3] (GTX101693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101693_IHC_19011506_638.jpg

GTX101693 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using SEPT2 (GTX101693) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101693_WB_19011506_536.jpg

GTX101693 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX101693 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169