Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody (GTX101774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody (GTX101774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Septin 2(GTX101774) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: U87-MG
B: SK-N-SH
10% SDS PAGE
GTX101774 diluted at 1:1000