APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/mL |
10 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide
Product Note
At least three isoforms of SPT2 are known to exist; this antibody will not detect isoform b. SPT2 antibody is predicted to not cross-react with SPT1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Serine palmitoyltransferase 2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Serine palmitoyltransferase 2.The immunogen is located within the last 50 amino acids of Serine palmitoyltransferase 2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine palmitoyltransferase long chain base subunit 2 , HSN1C , LCB2 , LCB2A , NSAN1C , SPT2 , hLCB2a
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
|
GTX31437 WB Image
WB analysis of 3T3 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31437 Serine palmitoyltransferase 2 antibody. Working concentration : 0.5 μg/ml
|
|
GTX31437 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX31437 Serine palmitoyltransferase 2 antibody. Working concentration : 20 μg/ml
|
|
GTX31437 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX31437 Serine palmitoyltransferase 2 antibody. Working concentration : 10 μg/ml
|
REFERENCE
There are currently no references for Serine palmitoyltransferase 2 antibody (GTX31437). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Serine palmitoyltransferase 2 antibody (GTX31437). Be the first to share your experience with this product.