GeneTex
United States (US)

Serine palmitoyltransferase 2 antibody

GTX31437_2633_WB_20180221_18121410_395.jpg
GTX31437_170_ICC-IF_20180221_18121410_131.jpg
GTX31437_171_ICC-IF_20180221_18121410_824.jpg

Cat No. GTX31437

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide

Specificity/Sensitivity

At least three isoforms of SPT2 are known to exist; this antibody will not detect isoform b. SPT2 antibody is predicted to not cross-react with SPT1.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Serine palmitoyltransferase 2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Serine palmitoyltransferase 2.The immunogen is located within the last 50 amino acids of Serine palmitoyltransferase 2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine Palmitoyltransferase Long Chain Base Subunit 2,Hsn1C,Lcb2,Lcb2A,Nsan1C,Spt2,Hlcb2A,Sptlc2

Background

This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011]

Database

DATA IMAGES
GTX31437_2633_WB_20180221_18121410_395.jpg

GTX31437 WB Image

WB analysis of 3T3 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31437 Serine palmitoyltransferase 2 antibody.
Working concentration : 0.5 μg/ml

GTX31437_170_ICC-IF_20180221_18121410_131.jpg

GTX31437 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31437 Serine palmitoyltransferase 2 antibody.
Working concentration : 10 μg/ml

GTX31437_171_ICC-IF_20180221_18121410_824.jpg

GTX31437 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31437 Serine palmitoyltransferase 2 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329