GeneTex
United States (US)

Serotonin Transporter antibody

GTX133101_42578_20180131_ICC_IF_R_18121410_289.jpg
GTX133101_42578_20161215_WB_R_intestine_18121410_745.jpg

Cat No. GTX133101

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

rat intestine

Predict Reactivity

Human, Mouse, Bovine, Guinea pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.84mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Serotonin Transporter. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

5HTT antibody , AI323329 antibody , Htt antibody , Sert antibody , Slc6a4 antibody , solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody

Background

Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adapter.

Database

Research Area

DATA IMAGES
GTX133101_42578_20180131_ICC_IF_R_18121410_289.jpg

GTX133101 ICC/IF Image

Serotonin Transporter antibody detects Serotonin Transporter protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Serotonin Transporter stained by Serotonin Transporter antibody (GTX133101) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133101_42578_20161215_WB_R_intestine_18121410_745.jpg

GTX133101 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Serotonin transporter antibody (GTX133101) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169