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Serotonin antibody [5-HT-H209]

Anti-Serotonin antibody [5-HT-H209] used in IHC (Paraffin sections) (IHC-P). GTX31099

Cat. No. GTX31099

Reference 10
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5-HT-H209

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Species independent
Package
100 μl ($399)
See all Serotonin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:50
IHC-P 1:50 - 1:100
IHC-Fr 1:50 - 1:100
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

Reacts with serotonin in a broad range of normal, hyperplastic and neoplastic tissues. Serotonin is also called 5-hydroxytryptamine. The antibody is of particular importance in identifying primary and metastatic carcinoid tumors expressing serotonin. This antibody may be a useful tool for characterization of carcinoids. In a study of 48 cases of adenomas and carcinoid tumours of the middle ear, the labelled tumour cells in 12 cases (25%).

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant dialysed against 0.05 mol/L Tris-HCl pH7.2

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Immunogen

5-Hydroxytryptamine hydrochloride (3-(2-aminoethyl)-5-hydroxyindole;serotonin).

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_10727660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Serotonin-positive cells are present in gastric, duodenal, jejunal, ileal, colonic, and appendiceal mucosae. Positive cells are sparse and usually occur in the lower half of the gastric and intestinal mucosae. In the appendix, in addition to the mucosal serotonin-containing cells, there are single or small clusters of serotonin-positive cells in the sub-epithelial region of the lamina propria.

Research Area

DATA IMAGES

Anti-Serotonin antibody [5-HT-H209] used in IHC (Paraffin sections) (IHC-P). GTX31099

GTX31099 IHC-P Image

IHC-P analysis of human appendix tissue using GTX31099 Serotonin antibody [5-HT-H209].

REFERENCE

Application Reference

Lin F et al. Brain Sci 2023; 13 (6) Maternal LPS Exposure Enhances the 5-HT Level in the Prefrontal Cortex of Autism-like Young Offspring.
Fang Lin et al. Brain Sci 2023; 13 (6) : 958 Maternal LPS Exposure Enhances the 5-HT Level in the Prefrontal Cortex of Autism-like Young Offspring
Application : IHC-Fr
Lu Y et al. Front Physiol 2022; 13 : 990178 Activation of uroepithelial 5-HT(4)R inhibits mechanosensory activity of murine bladder afferent nerves.
Liu XJ et al. Neurochem Int 2021; 150 : 105180 GABABR1 in DRN mediated GABA to regulate 5-HT expression in multiple brain regions in male rats with high and low aggressive behavior.
Application : IHC-P
G?k?e I??l Kurmu? et al. Ann Dermatol 2019; 31 (2) : 146-153 Serotonin Expression in Lichen Planus Lesions and Its Relationship with Depression/Anxiety.
Application : IHC-P
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REVIEW

Serotonin antibody [5-HT-H209] Cat. No. GTX31099

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Anti-Serotonin antibody [5-HT-H209] used in IHC (Whole mount) (IHC-Wm). GTX31099
Date : Anonymous submitted on 18-Apr-2022
Score :
Application Tested : IHC-Wm
Sample : The whole gills of Polypterus senegalus
Blocking : 10% NGS and 1% BSA in 0.7x PBS, 25°C, 1Hr
Fixation : 4% PFA in 0.7x PBS
Permeabilization /Antigen retrieval : 0.5% Triton x-100 (v/v) in 0.7x PBS
Primary Antibody : 1:500, 4°C, 18Hr
Figure Note : Green : Neuroepithelial cells (NECs) present in the gills were labeled.
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Application Reference
Lin F et al. Brain Sci 2023; 13 (6) Maternal LPS Exposure Enhances the 5-HT Level in the Prefrontal Cortex of Autism-like Young Offspring.
Fang Lin et al. Brain Sci 2023; 13 (6):958 Maternal LPS Exposure Enhances the 5-HT Level in the Prefrontal Cortex of Autism-like Young Offspring
Application : IHC-Fr
Lu Y et al. Front Physiol 2022; 13:990178 Activation of uroepithelial 5-HT(4)R inhibits mechanosensory activity of murine bladder afferent nerves.
Liu XJ et al. Neurochem Int 2021; 150:105180 GABABR1 in DRN mediated GABA to regulate 5-HT expression in multiple brain regions in male rats with high and low aggressive behavior.
Application : IHC-P
G?k?e I??l Kurmu? et al. Ann Dermatol 2019; 31 (2):146-153 Serotonin Expression in Lichen Planus Lesions and Its Relationship with Depression/Anxiety.
Application : IHC-P
Brokhman I et al. Dev Biol 2019; 445 (2):256-270 Dual embryonic origin of the mammalian enteric nervous system.
Eggermont M et al. Anat Rec (Hoboken) 2018; (Epub) Regional structural and functional specialisations in the urethra of the female rat: evidence for complex physiological control systems.
Application : IHC-Fr
Iwata K et al. Mol Autism 2014; 5:33 N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism.
Application : ICC/IF
Vela Hinojosa C et al. PLoS One 2013; 8 (9):e75062 Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
Application : IHC-Fr
Clone Reference
Becnel J et al. PLoS One 2011; 6 (6):e20800 The serotonin 5-HT7Dro receptor is expressed in the brain of Drosophila, and is essential for normal courtship and mating.
Application : IHC
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399