GeneTex
United States (US)

Serotonin antibody [5-HT-H209]

GTX31099_603_IHC-P_18121410_792.jpg

Cat No. GTX31099

Host Mouse
Clonality Monoclonal
Clone Name 5HT-H209
Isotype IgG1
Application ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Chicken, Pig, Rhesus Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:50
IHC-P 1:50 - 1:100
IHC-Fr 1:50 - 1:100
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Reacts with serotonin in a broad range of normal, hyperplastic and neoplastic tissues. Serotonin is also called 5-hydroxytryptamine. The antibody is of particular importance in identifying primary and metastatic carcinoid tumors expressing serotonin. This antibody may be a useful tool for characterization of carcinoids. In a study of 48 cases of adenomas and carcinoid tumours of the middle ear, the labelled tumour cells in 12 cases (25%).
PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant dialysed against 0.05 mol/L Tris/HCl pH 7.2, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Immunogen

5-Hydroxytryptamine hydrochloride (3-(2-aminoethyl)-5-hydroxyindole;serotonin).

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

5 hydroxytryptamine, 5HT, 25689, 5 HT5A, 5 HT, 5HT 15563, 5HT15563, 5 HT 5A

Cellular Localization

Cytoplasmic

Background

Serotonin-positive cells are present in gastric, duodenal, jejunal, ileal, colonic, and appendiceal mucosae. Positive cells are sparse and usually occur in the lower half of the gastric and intestinal mucosae. In the appendix, in addition to the mucosal serotonin-containing cells, there are single or small clusters of serotonin-positive cells in the sub-epithelial region of the lamina propria.

Research Area

DATA IMAGES
GTX31099_603_IHC-P_18121410_792.jpg

GTX31099 IHC-P Image

IHC-P analysis of human appendix tissue using GTX31099 Serotonin antibody [5-HT-H209].

REFERENCE
Package List Price ($)
$ 319