GeneTex
United States (US)

SerpinB3 antibody [N2C3]

Overexpression
GTX114739_43411_20181221_WB_19021719_487.jpg
GTX114739_43411_20190201_WB_B_19030801_823.jpg
GTX114739_40233_IFA_18121410_743.jpg
GTX114739_40233_IHC_18121410_179.jpg

Cat No. GTX114739

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SerpinB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serpin Family B Member 3,Hst1196,Scc,Scca-1,Scca-Pd,Scca1,Ssca1,T4-A,Serpinb3

Cellular Localization

Cytoplasm

Background

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Database

DATA IMAGES
GTX114739_43411_20181221_WB_19021719_487.jpg

GTX114739 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114739_43411_20190201_WB_B_19030801_823.jpg

GTX114739 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114739_40233_IFA_18121410_743.jpg

GTX114739 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using SerpinB3(GTX114739) antibody at 1:500 dilution.

GTX114739_40233_IHC_18121410_179.jpg

GTX114739 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using SerpinB3(GTX114739) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169