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SerpinB3 antibody [N2C3]

Overexpression
Anti-SerpinB3 antibody [N2C3] used in Western Blot (WB). GTX114739
Anti-SerpinB3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114739
Anti-SerpinB3 antibody [N2C3] used in Western Blot (WB). GTX114739
Anti-SerpinB3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114739

Cat. No. GTX114739

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SerpinB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , SERPINB3-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SerpinB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623421

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family B member 3 , HsT1196 , SCC , SCCA-1 , SCCA-PD , SCCA1 , SSCA1 , T4-A

Cellular Localization

Cytoplasm

Background

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Database

Research Area

DATA IMAGES

Anti-SerpinB3 antibody [N2C3] used in Western Blot (WB). GTX114739

GTX114739 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SerpinB3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114739

GTX114739 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using SerpinB3(GTX114739) antibody at 1:500 dilution.

Anti-SerpinB3 antibody [N2C3] used in Western Blot (WB). GTX114739

GTX114739 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SerpinB3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114739

GTX114739 IHC-P Image

SerpinB3 antibody [N2C3] detects SerpinB3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
SerpinB3 stained by SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169