APPLICATION
Application Note
For ELISA: Use at an assay dependent dilution. For WB: Use at a concentration of 0.1 - 0.5 μg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa). Optimal dilutions/concentrations should be determined by the researcher.
Product Note
Reacts with endogneous and recombinant human SAA. Does not cross-react with other human cytokines or growth factors tested such as IL1?, IL8, MCAF, TGF? and EGF.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Highly purified recombinant human SAA (MW: 12 kDa)
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Serum Amyloid A1 , Pig4 , Saa , Saa2 , Tp53I4 , Saa1
Cellular Localization
Secreted
Background
The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging. Expression studies show production of SAA proteins in histologically normal, atherosclerotic, Alzheimer, inflammatory, and tumour tissues. SAA has been found to have binding sites for high density lipoproteins, calcium, laminin, and heparin/heparin sulphate. Also adhesion motifs were identified and new functions affecting cell adhesion, migration, proliferation, and aggregation were discovered. These findings emphasize the importance of SAA in various physiological and pathological processes including inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia. SAA has also a number of immunomodulatory roles, it can induce chemotaxis and adhesion molecule expression, has cytokine-like properties and can promote the upregulation of metalloproteinases. It enhances the binding of high density lipoprotein to macrophages and thus helps in the delivery of lipids to sites of injury for use in tissue repair, it is thus thought to be an integral part of the disease process.
Research Area
REFERENCE
There are currently no references for Serum Amyloid A antibody [585] (GTX18792). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Serum Amyloid A antibody [585] (GTX18792). Be the first to share your experience with this product.