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Shh antibody [10H6]

Overexpression
Anti-Shh antibody [10H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83651
Anti-Shh antibody [10H6] used in Western Blot (WB). GTX83651
Anti-Shh antibody [10H6] used in Western Blot (WB). GTX83651

Cat. No. GTX83651

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10H6

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all Shh products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 24-197 of human SHH (NP_000184) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sonic hedgehog signaling molecule , HHG1 , HLP3 , HPE3 , MCOPCB5 , SMMCI , ShhNC , TPT , TPTPS

Background

Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.

Database

Research Area

DATA IMAGES

Anti-Shh antibody [10H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83651

GTX83651 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Shh plasmid using GTX83651 Shh antibody [10H6].

Anti-Shh antibody [10H6] used in Western Blot (WB). GTX83651

GTX83651 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Shh plasmid (Right) or empty vector (Left) for 48 hrs using GTX83651 Shh antibody [10H6].
Loading : 5 ug per lane

Anti-Shh antibody [10H6] used in Western Blot (WB). GTX83651

GTX83651 WB Image

WB analysis of various cell lines using GTX83651 Shh antibody [10H6].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519