APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sonic hedgehog signaling molecule , HHG1 , HLP3 , HPE3 , MCOPCB5 , SMMCI , ShhNC , TPT , TPTPS
Background
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right
Database
Research Area
DATA IMAGES
![Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606](/templates/default/js/lightbox/images/loading.gif "Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606")
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GTX60606 WB Image
WB analysis of LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate using GTX60606 Shh antibody [5H4].
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GTX60606 ELISA Image
ELISA analysis of antigen using GTX60606 Shh antibody [5H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60606 FACS Image
FACS analysis of HeLa cells using GTX60606 Shh antibody [5H4].
Green : Shh
Red : negative control
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GTX60606 IHC-P Image
IHC-P analysis of liver cancer tissue using GTX60606 Shh antibody [5H4].
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GTX60606 WB Image
WB analysis of HEK293 (1) and Shh (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate using GTX60606 Shh antibody [5H4].
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REFERENCE
There are currently no references for Shh antibody [5H4] (GTX60606). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Shh antibody [5H4] (GTX60606). Be the first to share your experience with this product.
![Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606](/upload/website/prouct_img/small/GTX60606/GTX60606_20170912_WB_1_w_23061123_408.webp "Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606")
![Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606](/upload/website/prouct_img/small/GTX60606/GTX60606_20170912_ELISA_w_23061123_608.webp "Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606")
![Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606](/upload/website/prouct_img/small/GTX60606/GTX60606_20170912_FACS_w_23061123_260.webp "Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606")
![Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606](/upload/website/prouct_img/small/GTX60606/GTX60606_20170912_IHC-P_w_23061123_688.webp "Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606")
![Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606](/upload/website/prouct_img/small/GTX60606/GTX60606_20170912_WB_w_23061123_360.webp "Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606")
![Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606](/upload/website/prouct_img/normal/GTX60606/GTX60606_20170912_WB_1_w_23061123_408.webp "Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606")
![Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606](/upload/website/prouct_img/normal/GTX60606/GTX60606_20170912_ELISA_w_23061123_608.webp "Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606")
![Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606](/upload/website/prouct_img/normal/GTX60606/GTX60606_20170912_FACS_w_23061123_260.webp "Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606")
![Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606](/upload/website/prouct_img/normal/GTX60606/GTX60606_20170912_IHC-P_w_23061123_688.webp "Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606")
![Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606](/upload/website/prouct_img/normal/GTX60606/GTX60606_20170912_WB_w_23061123_360.webp "Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606")