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Shh antibody [5H4]

Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606
Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606
Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606
Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606
Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606

Cat No. GTX60606

Host

Mouse

Clonality

Monoclonal

Clone Name

5H4

Isotype

IgG1

Application

WB, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sonic hedgehog signaling molecule , HHG1 , HLP3 , HPE3 , MCOPCB5 , SMMCI , ShhNC , TPT , TPTPS

Background

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right

Database

Research Area

DATA IMAGES

Anti-Shh antibody [5H4] used in Flow cytometry (FACS). GTX60606

GTX60606 FACS Image

FACS analysis of HeLa cells using GTX60606 Shh antibody [5H4].
Green : Shh
Red : negative control

Anti-Shh antibody [5H4] used in ELISA (ELISA). GTX60606

GTX60606 ELISA Image

ELISA analysis of antigen using GTX60606 Shh antibody [5H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606

GTX60606 WB Image

WB analysis of HEK293 (1) and Shh (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate using GTX60606 Shh antibody [5H4].

Anti-Shh antibody [5H4] used in Western Blot (WB). GTX60606

GTX60606 WB Image

WB analysis of LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate using GTX60606 Shh antibody [5H4].

Anti-Shh antibody [5H4] used in IHC (Paraffin sections) (IHC-P). GTX60606

GTX60606 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60606 Shh antibody [5H4].

REFERENCE

There are currently no references for Shh antibody [5H4] (GTX60606). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Shh antibody [5H4] (GTX60606). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339