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Shh antibody

Anti-Shh antibody used in Western Blot (WB). GTX37548
Anti-Shh antibody used in Western Blot (WB). GTX37548
Anti-Shh antibody used in IHC (Paraffin sections) (IHC-P). GTX37548

Cat No. GTX37548

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
50 μg ($229)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-2000
IHC-P 1:400-800
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M TBS pH7.4, 0.5% BSA, 25% Glycerol

Preservative

0.015% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived between 31-110 amino acids of human Sonic Hedgehog

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11173873

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sonic hedgehog signaling molecule , HHG1 , HLP3 , HPE3 , MCOPCB5 , SMMCI , ShhNC , TPT , TPTPS

Background

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Shh antibody used in Western Blot (WB). GTX37548

GTX37548 WB Image

WB analysis of human colon carcinoma tissue lysate using GTX37548 Shh antibody.
Dilution : 1:200

Anti-Shh antibody used in Western Blot (WB). GTX37548

GTX37548 WB Image

WB analysis of mouse liver tissue lysate using GTX37548 Shh antibody.

Anti-Shh antibody used in IHC (Paraffin sections) (IHC-P). GTX37548

GTX37548 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX37548 Shh antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 229