GeneTex
United States (US)

Siglec 7 antibody

Anti-Siglec 7 antibody used in Western Blot (WB). GTX107080
Anti-Siglec 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX107080
Anti-Siglec 7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107080

Cat No. GTX107080

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , NCI-H929
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Siglec 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sialic Acid Binding Ig Like Lectin 7,Airm1,Cd328,Cdw328,D-Siglec,Qa79,Siglec-7,Siglec19P,Siglecp2,P75,P75/Airm1,Siglec7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).

Database

Research Area

DATA IMAGES
Anti-Siglec 7 antibody used in Western Blot (WB). GTX107080

GTX107080 WB Image

Siglec 7 antibody detects Siglec 7 protein by western blot analysis.
A. 30 µg K562 whole cell lysate/extract
B. 30 µg THP-1 whole cell lysate/extract
C. 30 µg HL-60 whole cell lysate/extract
D. 30 µg NCI-H929 whole cell lysate/extract
10% SDS-PAGE
Siglec 7 antibody (GTX107080) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Siglec 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX107080

GTX107080 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Siglec 7(GTX107080) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Siglec 7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107080

GTX107080 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Siglec 7(GTX107080) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169