GeneTex
United States (US)

Smad1 (phospho Ser187) antibody

GTX32213_20190131_IHC-P_19013102_312.jpg
GTX32213_20190131_WB_19013102_389.jpg

Cat No. GTX32213

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Smad1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Smad Family Member 1,Bsp-1,Bsp1,Jv4-1,Jv41,Madh1,Madr1,Smad1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX32213_20190131_IHC-P_19013102_312.jpg

GTX32213 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32213 Smad1 (phospho Ser187) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32213_20190131_WB_19013102_389.jpg

GTX32213 WB Image

WB analysis of BMP4-treated HEK293T (A), PMA-treated HeLa(B), A673 (C), PC12 (D) whole cell lysates using GTX32213 Smad1 (phospho Ser187) antibody.

Package List Price ($)
$ 319