GeneTex
United States (US)

Smad5 antibody

GTX124559_40464_IHC_Z_18121410_699.jpg
GTX124559_40464_20150626_WB_Z_tissue_18121410_485.jpg

Cat No. GTX124559

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

zebrafish brain , zebrafish heart , zebrafish liver , zebrafish muscle
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Smad5

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Smad Family Member 5,Sbn,Fb67B04,Mad,Madh5,Pgy,Sb:Cb400,Smd5,Wu:Fb67B04,Smad5

Background

May be involved in apoptosis regulation.

Research Area

DATA IMAGES
GTX124559_40464_IHC_Z_18121410_699.jpg

GTX124559 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using smad5 (GTX124559) antibody at 1:300 dilution.

GTX124559_40464_20150626_WB_Z_tissue_18121410_485.jpg

GTX124559 WB Image

smad5 antibody detects smad5 protein by western blot analysis. Various zebrafish tissue extracts (30 μg) were separated by 10 % SDS-PAGE, and the membrane was blotted with smad5 antibody (GTX124559) at a dilution of 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199