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Smad5 antibody

Anti-Smad5 antibody used in IHC (Paraffin sections) (IHC-P). GTX124559
Anti-Smad5 antibody used in Western Blot (WB). GTX124559

Cat No. GTX124559

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

zebrafish brain , zebrafish heart , zebrafish liver , zebrafish muscle

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Smad5

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 5 , SBN , fb67b04 , mad , madh5 , pgy , sb:cb400 , smd5 , wu:fb67b04

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Smad5 antibody used in IHC (Paraffin sections) (IHC-P). GTX124559

GTX124559 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using smad5 (GTX124559) antibody at 1:300 dilution.

Anti-Smad5 antibody used in Western Blot (WB). GTX124559

GTX124559 WB Image

smad5 antibody detects smad5 protein by western blot analysis. Various zebrafish tissue extracts (30 μg) were separated by 10 % SDS-PAGE, and the membrane was blotted with smad5 antibody (GTX124559) at a dilution of 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219