Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SMARCAL1 protein by 4 μg SmarcAL1 antibody (GTX109468)
5 % SDS-PAGE
The immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody (GTX109468) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SmarcAL1(GTX109468) antibody at 1:500 dilution.