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SmarcAL1 antibody

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468
Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468
Anti-SmarcAL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109468
Anti-SmarcAL1 antibody used in Immunoprecipitation (IP). GTX109468
Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468

Cat No. GTX109468

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

A549 , mouse testis , rat testis

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SmarcAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179132

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1 , HARP , HHARP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468

GTX109468 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468

GTX109468 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SmarcAL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109468

GTX109468 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SmarcAL1(GTX109468) antibody at 1:500 dilution.

Anti-SmarcAL1 antibody used in Immunoprecipitation (IP). GTX109468

GTX109468 IP Image

SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SMARCAL1 protein by 4 μg SmarcAL1 antibody (GTX109468)
5 % SDS-PAGE
The immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody (GTX109468) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX109468

GTX109468 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169