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SmarcAL1 antibody

Orthogonal
Anti-SmarcAL1 antibody used in Western Blot (WB). GTX24743
Anti-SmarcAL1 antibody used in Western Blot (WB). GTX24743

Cat. No. GTX24743

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all SmarcAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:16000-1:64000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal near aa 75-100 of human SmarcAL1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1 , HARP , HHARP

Cellular Localization

Nucleus

Background

This protein is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Database

Research Area

DATA IMAGES

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX24743

GTX24743 WB Image

Western blot using GeneTex's Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kDa band corresponding to SmarcAL1 in human derived cultured cell lysates (Lane1: HeLa nuclear extract, Lane2: HeLa cells, Lane3: A431 celss, lane 4: Jurkat cells, Lane5: 293T cells). Approximately 5μg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.

Anti-SmarcAL1 antibody used in Western Blot (WB). GTX24743

GTX24743 WB Image

Orthogonal

WB analysis of various samples using GTX24743 SmarcAL1 antibody.
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : 293T whole cell lysate
Loading : 5 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399