WB analysis of various samples using GTX24743 SmarcAL1 antibody.
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : 293T whole cell lysate
Loading : 5 μg
Dilution : 1:500

Western blot using GeneTex's Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kDa band corresponding to SmarcAL1 in human derived cultured cell lysates (Lane1: HeLa nuclear extract, Lane2: HeLa cells, Lane3: A431 celss, lane 4: Jurkat cells, Lane5: 293T cells). Approximately 5μg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.