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Smarcb1a antibody

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337
Anti-Smarcb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124337
Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337
Anti-Smarcb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124337

Cat No. GTX124337

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
200 μl ($409),
50 μl ($219)
See all zebrafish Smarcb1a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

adult zebrafish

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1a , zgc:92517

Cellular Localization

Nucleus

Background

Involved in chromatin-remodeling. Part of a complex that opens the chromatin to facilitate the transcriptional machinery to access their targets. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR).

Database

Research Area

DATA IMAGES

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337

GTX124337 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Smarcb1a antibody (GTX124337) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Smarcb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124337

GTX124337 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using smarcb1a (GTX124337) antibody at 1:300 dilution.

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337

GTX124337 WB Image

Sample (10 ug of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
GTX124337 diluted at 1:1000

Anti-Smarcb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124337

GTX124337 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using smarcb1a (GTX124337) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219