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Smarcb1a antibody

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337
Anti-Smarcb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124337
Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337
Anti-Smarcb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124337

Cat. No. GTX124337

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

adult zebrafish

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Bovine (100%)| Chicken (100%)| Xenopus tropicalis (99%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Smarcb1a

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179037

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1a , zgc:92517

Cellular Localization

Nucleus

Background

Involved in chromatin-remodeling. Part of a complex that opens the chromatin to facilitate the transcriptional machinery to access their targets. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR).

Database

Research Area

DATA IMAGES

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337

GTX124337 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Smarcb1a antibody (GTX124337) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Smarcb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124337

GTX124337 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using smarcb1a (GTX124337) antibody at 1:200 dilution.

Anti-Smarcb1a antibody used in Western Blot (WB). GTX124337

GTX124337 WB Image

Sample (10 ug of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
GTX124337 diluted at 1:1000

Anti-Smarcb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124337

GTX124337 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using smarcb1a (GTX124337) antibody at 1:300 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169