Smoothened homolog antibody [HL2975]

Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Smoothened homolog antibody [HL2975] (GTX640362) detects Smoothened homolog protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4ºC for 1 hour.

Smoothened homolog antibody [HL2975] (GTX640362) specifically detects Smoothened homolog protein on a VirD™-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:2000 dilution.
Yellow: SMO

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Boiled and unboiled mouse brain extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.