GeneTex
United States (US)

SnoN antibody

GTX16977_701_IHC-P_20180221_18121410_366.jpg
GTX16977_22_IHC-P_20180221_18121410_734.jpg
GTX16977_2301_WB_20180221_18121410_875.jpg

Cat No. GTX16977

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A431 Cell Lysate(GTX27909) , Mouse Lung Tissue Lysate(GTX27933)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN.The immunogen is located within the first 50 amino acids of SnoN.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ski Like Proto-Oncogene,Sno,Snoa,Snoi,Snon,Skil

Background

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES
GTX16977_701_IHC-P_20180221_18121410_366.jpg

GTX16977 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX16977 SnoN antibody.
Working concentration : 5 μg/ml

GTX16977_22_IHC-P_20180221_18121410_734.jpg

GTX16977 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX16977 SnoN antibody.
Working concentration : 20 μg/ml

GTX16977_2301_WB_20180221_18121410_875.jpg

GTX16977 WB Image

WB analysis of A431 cell lysate using GTX16977 SnoN antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml