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Sod2 antibody

Anti-Sod2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124294
Anti-Sod2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124294
Anti-Sod2 antibody used in Western Blot (WB). GTX124294

Cat. No. GTX124294

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Sod2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:50-1:500
Not tested in other applications.

Positive Control

zebrafish liver , zebrafish GI , whole zebrafish

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Sod2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174816

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2, mitochondrial , cb463 , wu:fj33b01 , zgc:73051

Database

Research Area

DATA IMAGES

Anti-Sod2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124294

GTX124294 IHC-Wm Image

Sod2 antibody detects Sod2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sod2 antibody (GTX124294) dilution: 1:50.
Anti-Sod2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124294

GTX124294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney ducts, using sod2 (GTX124294) antibody at 1:300 dilution.

Anti-Sod2 antibody used in Western Blot (WB). GTX124294

GTX124294 WB Image

Various tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Sod2 antibody (GTX124294) diluted at 1:1000. (GI: gastrointestinal)

REFERENCE

Application Reference

G Orso et al. Environments 2023; 10 (12) : 219 A Deep Survey of Fish Health for the Recognition of Useful Biomarkers to Monitor Water Pollution
Ding Q et al. iScience 2021; 24 (6) : 102515 Propionate induces intestinal oxidative stress via Sod2 propionylation in zebrafish.
Application : WB
Eason J et al. Birth Defects Res 2017; Differences in neural crest sensitivity to ethanol account for the infrequency of anterior segment defects in the eye compared with craniofacial anomalies in a zebrafish model of fetal alcohol syndrome.
Hallauer J et al. Zebrafish 2016; (Epub) The Effect of Chronic Arsenic Exposure in Zebrafish.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169