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Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)]

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in IHC (Paraffin sections) (IHC-P). GTX30202
Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202
Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Western Blot (WB). GTX30202
Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202
Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202

Cat. No. GTX30202

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 464.6(6H)

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Yeast, Rabbit, Drosophila, Bovine, Dog, Guinea pig, Pig, Xenopus, Primate
Package
50 μl ($439)
See all Sodium/Potassium ATPase alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
ICC/IF 1:50 - 1:1000
IHC-P 1:200
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in IHC (Paraffin sections) (IHC-P). GTX30202

GTX30202 IHC-P Image

IHC-P analysis of human uterus tissue using GTX30202 Sodium Potassium ATPase Alpha 1 antibody [464.6(6H)].

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202

GTX30202 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30202 Sodium Potassium ATPase Alpha 1 antibody [464.6(6H)].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 10 μg/ml
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Western Blot (WB). GTX30202

GTX30202 WB Image

WB analysis of pig proximal tubule protein using GTX30202 Sodium Potassium ATPase Alpha 1 antibody [464.6(6H)].

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202

GTX30202 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX30202 Sodium Potassium ATPase Alpha 1 antibody [464.6(6H)].
Green : primary antibody
Blue : Hoechst 33258

Anti-Sodium/Potassium ATPase alpha 1 antibody [464.6(6H)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30202

GTX30202 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX30202 Sodium Potassium ATPase Alpha 1 antibody [464.6(6H)].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 10 μg/ml
Fixation : 10% formalin
Permibilization : 1X TBS + 0.5% Triton-X100 for 5 minutes

REFERENCE

Application Reference

Guillermo F. et al. bioRxiv 2021; (Epub) A New Old Tale: Dopamine Transporter implications in an animal model of Attention-Deficit Hyperactivity Disorder.
Gentil C et al. Hum Gene Ther 2016; (Epub) Dystrophin threshold level necessary for normalisation of nNOS, iNOS and RyR1 nitrosylation in GRMD dystrophinopathy.
Application : WB
Reactivity : Dog
Durieux AC et al. Traffic 2012; 13 (6) : 869-79 A centronuclear myopathy--dynamin 2 mutation impairs autophagy in mice.
Kapri-Pardes E et al. J Biol Chem 2011; 286 (50) : 42888-99 Stabilization of the α2 isoform of Na,K-ATPase by mutations in a phospholipid binding pocket.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 439