Sodium/Potassium ATPase alpha 1 antibody [HL114]

Name: Sodium/Potassium ATPase alpha 1 antibody [HL114]
Brand: GeneTex
SKU: GTX635461
Price: 399.00 USD

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Western Blot (WB). GTX635461

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635461

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in IHC (Paraffin sections) (IHC-P). GTX635461

Cat. No. GTX635461

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL114
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all Sodium/Potassium ATPase alpha 1 products

Datasheet
Reference

View product citations for antibody GTX635461 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

The immunogen used to generate this antibody corresponds to chicken Sodium/Potassium ATPase alpha 1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888530

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1

Database

Gene ID: 396530 ATP1A1
UniProt: P09572 ATP1A1

Research Area

DATA IMAGES

GTX635461 WB Image

A431 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX635461 WB Image

A431 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635461 ICC/IF Image

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635461 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635461 IHC-P Image

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635461 WB Image

Drosophila tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635461 IHC-P Image

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635461 IHC-P Image

The data was published in the 2022 in Nat Commun. PMID: 35618735

REFERENCE

Application Reference

Yi-Ing Chen et al. Nat Commun 2022; 13 (1) : 2945 Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

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REVIEW

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Application Reference

Yi-Ing Chen et al. Nat Commun 2022; 13 (1):2945 Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Sodium/Potassium ATPase alpha 1 antibody [HL114]

Cat. No. GTX635461

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Database

Research Area

DATA IMAGES

GTX635461 WB Image

GTX635461 WB Image

GTX635461 ICC/IF Image

GTX635461 WB Image

GTX635461 IHC-P Image

GTX635461 WB Image

GTX635461 IHC-P Image

GTX635461 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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