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Sodium/Potassium ATPase alpha 1 antibody [HL114]

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Western Blot (WB). GTX635461
Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in IHC (Paraffin sections) (IHC-P). GTX635461
Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635461
Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Western Blot (WB). GTX635461

Cat No. GTX635461

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL114

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431 , A431 membrane extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

The immunogen used to generate this antibody corresponds to chicken Sodium/Potassium ATPase alpha 1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888530

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Western Blot (WB). GTX635461

GTX635461 WB Image

A431 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in IHC (Paraffin sections) (IHC-P). GTX635461

GTX635461 IHC-P Image

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635461

GTX635461 ICC/IF Image

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Sodium/Potassium ATPase alpha 1 antibody [HL114] used in Western Blot (WB). GTX635461

GTX635461 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 369
$ 169