Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9]

Cat. No. GTX22871

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	M7-PB-E9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP, ELISA, IHC
Reactivity	Human, Mouse, Rat, Sheep, Bovine, Dog, Chicken, Pig
Package	200 μl ($399)

See all Sodium/Potassium ATPase alpha 1 products

View product citations for antibody GTX22871 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:5000
ICC/IF	1:20
IHC-P	1:100
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Product Note

This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase. In rat, this antibody can only detects the alpha 3 isoform.

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Sheep

Immunogen

Purified alpha sodium/potassium ATPase from sheep kidney.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384890

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Database

Gene ID: 443381 ATP1A1
UniProt: P04074 ATP1A1

Research Area

DATA IMAGES

GTX22871 IHC-P Image

IHC-P analysis of human testis tissue using GTX22871 Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX22871 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22871 Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

GTX22871 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22871 Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX22871 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22871 Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

GTX22871 WB Image

WB analysis of 25 ug of U251 (lane 1), human kidney (lane 2) and mouse kidney (lane 3) using GTX22871 Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9].
Dilution : 1:1000

REFERENCE

Application Reference

Nishiya N et al. Chem Biol 2014; A Zebrafish Chemical Suppressor Screening Identifies Small Molecule Inhibitors of the Wnt/β-catenin Pathway.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW

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Application Reference

Nishiya N et al. Chem Biol 2014; A Zebrafish Chemical Suppressor Screening Identifies Small Molecule Inhibitors of the Wnt/β-catenin Pathway.

Application : WB

Reactivity : Human

SDS
Sodium Azide.pdf

Package

List Price ($)

200 μl

$ 399

Qty

Sodium/Potassium ATPase alpha 1 antibody [M7-PB-E9]

Cat. No. GTX22871

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX22871 IHC-P Image

GTX22871 ICC/IF Image

GTX22871 IHC-P Image

GTX22871 IHC-P Image

GTX22871 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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