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Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22872
Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in Western Blot (WB). GTX22872
Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872
Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872
Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872

Cat. No. GTX22872

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 M8-P1-A3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Yeast, Sheep, Dog, Pig, Primate
Package
100 μg ($399)
See all Sodium/Potassium ATPase alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1/50 - 1/200
IHC-P 1/100 - 1/2000
IHC-Fr 1:100
FACS 1/20 - 1/100
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Product Note

This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sheep

Immunogen

Lamb kidney alpha 1 sodium/potassium ATPase.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384891

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1 , CMT2DD , HOMGSMR2

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22872

GTX22872 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 for at least 1 hour at room temperature

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in Western Blot (WB). GTX22872

GTX22872 WB Image

WB analysis of 25 ug of rat kidney (lane 1) and mouse kidney (lane 2) using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3].
Dilution : 1:1000

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872

GTX22872 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872

GTX22872 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3] used in IHC (Paraffin sections) (IHC-P). GTX22872

GTX22872 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

Application Reference

Reinhard JR et al. Nat Commun 2016; 7 : 11613 The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399