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Sodium/Potassium ATPase alpha 1 antibody

Anti-Sodium/Potassium ATPase alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64425
Anti-Sodium/Potassium ATPase alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64425
Anti-Sodium/Potassium ATPase alpha 1 antibody used in Western Blot (WB). GTX64425

Cat. No. GTX64425

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP1A1 (NP_000692.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1 , CMT2DD , HOMGSMR2

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64425

GTX64425 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64425 Sodium/Potassium ATPase alpha 1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Sodium/Potassium ATPase alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64425

GTX64425 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64425 Sodium/Potassium ATPase alpha 1 antibody.
Dilution : 1:100

Anti-Sodium/Potassium ATPase alpha 1 antibody used in Western Blot (WB). GTX64425

GTX64425 WB Image

WB analysis of various sample lysates using GTX64425 Sodium/Potassium ATPase alpha 1 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399