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Sodium/Potassium ATPase alpha 2 antibody

Anti-Sodium/Potassium ATPase alpha 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03457
Anti-Sodium/Potassium ATPase alpha 2 antibody used in Western Blot (WB). GTX03457
Anti-Sodium/Potassium ATPase alpha 2 antibody used in Western Blot (WB). GTX03457

Cat. No. GTX03457

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($599)
See all Sodium/Potassium ATPase alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
ICC/IF Assay dependent
IHC-Fr 1:300
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)REYSPAATTAENG, corresponding to amino acid residues 7-19 of rat ATP1A2 (Accession P06686). Intracellular, N-terminus.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit alpha 1 , Nkaa1b

Cellular Localization

Basolateral cell membrane , sarcolemma , axon , Melanosome

Background

mediates Na+ and K+ transport; may play a role in regulation of blood pressure [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase alpha 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03457

GTX03457 IHC-Fr Image

IHC-Fr analysis of mouse hippocampus and cerebellum tissues using GTX03457 Sodium/Potassium ATPase alpha 2 antibody.
Panel A : ATP1A2 staining (green, 1:300) in mouse hippocampal CA3 region is labeled in astrocytic processes (arrows surrounding pyramidal cells, P) and diffused staining is observed in the CA3 stratum radiatum. Note absence of staining in the mossy fiber terminal region (MF).
Panel B : Staining in mouse cerebellum shows labeling of the basket shape (arrows) under Purkinje cells (P) and diffused staining in the molecular layer (MOL).

Anti-Sodium/Potassium ATPase alpha 2 antibody used in Western Blot (WB). GTX03457

GTX03457 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membrane lysates using GTX03457 Sodium/Potassium ATPase alpha 2 antibody.
Lane 1, 2 : Primary antibody
Lane 3, 4 : Primary antibody preincubated with immunogen peptide
Dilution : 1:200

Anti-Sodium/Potassium ATPase alpha 2 antibody used in Western Blot (WB). GTX03457

GTX03457 WB Image

WB analysis of U-87 MG whole cell lysate using GTX03457 Sodium/Potassium ATPase alpha 2 antibody.
Lane 1 : Primary antibody
Lane 2 : Primary antibody preincubated with immunogen peptide
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 599