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Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11]

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873
Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873
Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in Western Blot (WB). GTX22873
Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22873
Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873

Cat. No. GTX22873

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 M17-P5-F11

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Sheep, Drosophila, Dog, Pig
Package
100 μl ($399)
See all Sodium/Potassium ATPase beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10,000
ICC/IF 1:100-1:1000
IHC-P 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sheep

Immunogen

Purified lamb kidney beta sodium/potassium ATPase.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384892

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 1 , ATP1B

Cellular Localization

Cell membrane

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873

GTX22873 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22873 Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873

GTX22873 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22873 Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in Western Blot (WB). GTX22873

GTX22873 WB Image

WB analysis of 25 ug of human brain (lane 1), human liver (lane 2), human kidney (lane 3) and mouse kidney (lane 4) lysates using GTX22873 Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11].
Dilution : 1:5000

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22873

GTX22873 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22873 Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11] used in IHC (Paraffin sections) (IHC-P). GTX22873

GTX22873 IHC-P Image

IHC-P analysis of human liver tissue using GTX22873 Sodium/Potassium ATPase beta 1 antibody [M17-P5-F11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

Application Reference

Gentil C et al. Hum Gene Ther 2016; (Epub) Dystrophin threshold level necessary for normalisation of nNOS, iNOS and RyR1 nitrosylation in GRMD dystrophinopathy.
Application : WB
Reactivity : Dog
Persson AK et al. J Neurosci 2013; 33 (49) : 19250-61 Sodium channels contribute to degeneration of dorsal root ganglion neurites induced by mitochondrial dysfunction in an in vitro model of axonal injury.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399