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Sodium/Potassium ATPase beta 1 antibody

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

Cat No. GTX113390

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

K562 , HL-60 , mouse brain , Rat brain

Predict Reactivity

Rabbit, Sheep, Dog, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sodium/Potassium ATPase beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036293

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 1 , ATP1B

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Human brain (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase beta 1 antibody (GTX113390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX113390 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ATPase beta1(Na+/K+)(GTX113390) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113390 diluted at 1:1000

REFERENCE

REVIEW

Sodium/Potassium ATPase beta 1 antibody Cat No. GTX113390

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24663680
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24663680
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2019.PMID: 31136617
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169