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Sodium/Potassium ATPase beta 1 antibody

Orthogonal
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

Cat. No. GTX113390

Reference 12
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Sodium/Potassium ATPase beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Rat brain , mouse brain

Predict Reactivity

Rabbit, Sheep, Dog, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sodium/Potassium ATPase beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 1 , ATP1B

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Human brain (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase beta 1 antibody (GTX113390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ATPase beta1(Na+/K+)(GTX113390) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113390

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX113390 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390

GTX113390 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113390 diluted at 1:1000

REFERENCE

Application Reference

Wu SY et al. Autophagy 2023; 19 (4) : 1239-1257 Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.
Application : WB
Reactivity : Mouse
Eid S et al. Int J Mol Sci 2022; 23 (23) Identification of a Cardiac Glycoside Exhibiting Favorable Brain Bioavailability and Potency for Reducing Levels of the Cellular Prion Protein.
Application : WB
Reactivity : Human
Williams D et al. PLoS One 2021; 16 (11) : e0258682 The cellular prion protein interacts with and promotes the activity of Na,K-ATPases.
Application : WB
Reactivity : Mouse
Solarski M et al. PLoS One 2019; 14 (5) : e0217392 The human brain somatostatin interactome: SST binds selectively to P-type family ATPases.
Application : WB
Reactivity : Human
Tokhtaeva E et al. J Cell Sci 2016; (Epub) FXYD5 O-glycosylated ectodomain impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits.
Application : WB
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

Sodium/Potassium ATPase beta 1 antibody Cat. No. GTX113390

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24663680
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24663680
Anti-Sodium/Potassium ATPase beta 1 antibody used in Western Blot (WB). GTX113390
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2019. PMID: 31136617
Submit a Review
Application Reference
Wu SY et al. Autophagy 2023; 19 (4):1239-1257 Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.
Application : WB
Reactivity : Mouse
Eid S et al. Int J Mol Sci 2022; 23 (23) Identification of a Cardiac Glycoside Exhibiting Favorable Brain Bioavailability and Potency for Reducing Levels of the Cellular Prion Protein.
Application : WB
Reactivity : Human
Williams D et al. PLoS One 2021; 16 (11):e0258682 The cellular prion protein interacts with and promotes the activity of Na,K-ATPases.
Application : WB
Reactivity : Mouse
Solarski M et al. PLoS One 2019; 14 (5):e0217392 The human brain somatostatin interactome: SST binds selectively to P-type family ATPases.
Application : WB
Reactivity : Human
Tokhtaeva E et al. J Cell Sci 2016; (Epub) FXYD5 O-glycosylated ectodomain impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits.
Application : WB
Reactivity : Rat
Flodby P et al. Am J Respir Cell Mol Biol 2016; (Epub) Knockout Mice Reveal a Major Role for Alveolar Epithelial Type I Cells in Alveolar Fluid Clearance.
Application : WB
Reactivity : Mouse
Kage H et al. Am J Physiol Lung Cell Mol Physiol 2014; 307 (7):L524-36 Claudin 4 knockout mice: normal physiological phenotype with increased susceptibility to lung injury.
Application : WB
Reactivity : Mouse
Kage H et al. Am J Physiol Lung Cell Mol Physiol 2014; ([Epub ahead of print]) Claudin 4 knockout mice: normal physiologic phenotype with increased susceptibility to lung injury
Application : WB
Reactivity : Mouse
Ding WG et al. PLoS One 2014; 9 (3):e92923 Improved functional expression of human cardiac kv1.5 channels and trafficking-defective mutants by low temperature treatment.
Application : WB
Reactivity : Human
Li G et al. Am J Respir Cell Mol Biol 2014; Knockout mice reveal key roles for claudin 18 in alveolar barrier properties and fluid homeostasis.
Application : WB
Reactivity : Mouse
Wen X et al. Eur J Oral Sci 2014; 122 (1):43272 Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.
Application : IHC-P
Reactivity : Rat
Marchelletta RR et al. Gastroenterology 2013; 145 (6):1358-1368.e1-4 Altered expression and localization of ion transporters contribute to diarrhea in mice with Salmonella-induced enteritis.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169