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Sodium/Potassium ATPase beta 3 antibody [N2C3]

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114272
Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Western Blot (WB). GTX114272

Cat No. GTX114272

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sodium/Potassium ATPase beta 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620114

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 3 , ATPB-3 , CD298

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114272

GTX114272 IHC-P Image

ATPase beta3 (Na+/K+) antibody [N2C3] detects ATPase beta3 (Na+/K+) protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATPase beta3 (Na+/K+) antibody [N2C3] (GTX114272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Western Blot (WB). GTX114272

GTX114272 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
ATPase beta3(Na+/K+) antibody
GTX114272 diluted at 1:1000

REFERENCE

REVIEW

Sodium/Potassium ATPase beta 3 antibody [N2C3] Cat No. GTX114272

Rating ( Average 5 based on 1 users reviews)
Application
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2020.PMID: 32575420
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169