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Sodium/Potassium ATPase beta 3 antibody [N2C3]

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114272
Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Western Blot (WB). GTX114272
Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Immunohistochemistry (IHC). GTX114272

Cat. No. GTX114272

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Sodium/Potassium ATPase beta 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Sodium Potassium ATPase Beta 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620114

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 3 , ATPB-3 , CD298

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114272

GTX114272 IHC-P Image

ATPase beta3 (Na+/K+) antibody [N2C3] detects ATPase beta3 (Na+/K+) protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATPase beta3 (Na+/K+) antibody [N2C3] (GTX114272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Western Blot (WB). GTX114272

GTX114272 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
ATPase beta3(Na+/K+) antibody
GTX114272 diluted at 1:1000

Anti-Sodium/Potassium ATPase beta 3 antibody [N2C3] used in Immunohistochemistry (IHC). GTX114272

GTX114272 IHC Image

The data was published in the journal Cancers (Basel) in 2020.PMID: 32575420

REFERENCE

Application Reference

Boral D et al. Cancers (Basel) 2020; 12 (6) Molecular Interplay between Dormant Bone Marrow-Resident Cells (BMRCs) and CTCs in Breast Cancer.
Wang S et al. Exp Ther Med 2017; 14 (2) : 1212-1218 connexin 43 enhances * cytotoxicity in colorectal cancer cell lines.
Application : WB
Reactivity : Human
Wang S et al. Exp Ther Med 2017; (Epub) connexin 43 enhances * cytotoxicity in colorectal cancer cell lines.
Wang Y et al. Analyst 2016; 141 (11) : 3411-20 investigation of an spr biosensor for determining the influence of connexin 43 expression on the cytotoxicity of *.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169