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Somatostatin antibody

Anti-Somatostatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133119
Anti-Somatostatin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119
Anti-Somatostatin antibody used in Western Blot (WB). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

Cat. No. GTX133119

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Somatostatin products
PRODUCT

Summary

Somatostatin antibody recognizes somatostatin, a small peptide hormone (existing in two forms) found predominately in the nervous and digestive systems. It acts as both a neurotransmitter and an inhibitory regulator of the secretion of other hormones. Somatostatin also has antiproliferative activity and promotes apoptosis.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Somatostatin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2814698

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

somatostatin , SOM , SRIF , SS , Smst

Cellular Localization

Secreted

Background

Somatostatin inhibits the release of somatotropin.

Database

Research Area

DATA IMAGES

Anti-Somatostatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133119

GTX133119 ICC/IF Image

Somatostatin antibody detects Somatostatin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Somatostatin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133119

GTX133119 IHC-Fr Image

Somatostatin antibody detects Somatostatin Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects secreted Somatostatin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin antibody used in Western Blot (WB). GTX133119

GTX133119 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Somatostatin antibody (GTX133119) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects Somatostatin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects Somatostatin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Miyamoto Y et al. eNeuro 2022; 9 (4) New Subregions of the Mouse Entopeduncular Nucleus Defined by the Complementary Immunoreactivities for Substance P and Cannabinoid Type-1 Receptor Combined with Distributions of Different Neuronal Types.
Fangbing Shao et al. Front Neurosci 2021; 15 : 691931 Electroacupuncture Ameliorates Chronic Inflammatory Pain-Related Anxiety by Activating PV Interneurons in the Anterior Cingulate Cortex.
Application : IHC-Fr
Reactivity : Rat
Lechner CR et al. Neurobiol Dis 2021; 148 : 105222 Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.
Reactivity : Mouse
Tran CG et al. Surgery 2020; Metastatic pancreatic neuroendocrine tumors have decreased somatostatin expression and increased Akt signaling.
Application : ICC/IF
Reactivity : Human
Chavez-Valdez R et al. J Cereb Blood Flow Metab 2020; : 271678X20942707 Accumulation of PSA-NCAM marks nascent neurodegeneration in the dorsal hippocampus after neonatal hypoxic-ischemic brain injury in mice.
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169