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Somatostatin antibody

Anti-Somatostatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133119
Anti-Somatostatin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119
Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119
Anti-Somatostatin antibody used in Western Blot (WB). GTX133119

Cat. No. GTX133119

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Somatostatin products
PRODUCT

Summary

Somatostatin antibody recognizes somatostatin, a small peptide hormone (existing in two forms) found predominately in the nervous and digestive systems. It acts as both a neurotransmitter and an inhibitory regulator of the secretion of other hormones. Somatostatin also has antiproliferative activity and promotes apoptosis.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Somatostatin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2814698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

somatostatin , SOM , SRIF , SS , Smst

Cellular Localization

Secreted

Background

Somatostatin inhibits the release of somatotropin.

Database

Research Area

DATA IMAGES

Anti-Somatostatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133119

GTX133119 ICC/IF Image

Somatostatin antibody detects Somatostatin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Somatostatin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133119

GTX133119 IHC-Fr Image

Somatostatin antibody detects Somatostatin Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects secreted Somatostatin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects Somatostatin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin antibody used in IHC (Paraffin sections) (IHC-P). GTX133119

GTX133119 IHC-P Image

Somatostatin antibody detects Somatostatin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Somatostatin stained by Somatostatin antibody (GTX133119) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin antibody used in Western Blot (WB). GTX133119

GTX133119 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Somatostatin antibody (GTX133119) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Miyamoto Y et al. eNeuro 2022; 9 (4) New Subregions of the Mouse Entopeduncular Nucleus Defined by the Complementary Immunoreactivities for Substance P and Cannabinoid Type-1 Receptor Combined with Distributions of Different Neuronal Types.
Fangbing Shao et al. Front Neurosci 2021; 15 : 691931 Electroacupuncture Ameliorates Chronic Inflammatory Pain-Related Anxiety by Activating PV Interneurons in the Anterior Cingulate Cortex.
Application : IHC-Fr
Reactivity : Rat
Lechner CR et al. Neurobiol Dis 2021; 148 : 105222 Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.
Reactivity : Mouse
Tran CG et al. Surgery 2020; Metastatic pancreatic neuroendocrine tumors have decreased somatostatin expression and increased Akt signaling.
Application : ICC/IF
Reactivity : Human
Chavez-Valdez R et al. J Cereb Blood Flow Metab 2020; : 271678X20942707 Accumulation of PSA-NCAM marks nascent neurodegeneration in the dorsal hippocampus after neonatal hypoxic-ischemic brain injury in mice.
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169