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Somatostatin receptor 3 antibody [HL2681]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345
Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345
Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345
Anti-Somatostatin receptor 3 antibody [HL2681] used in IHC (Paraffin sections) (IHC-P). GTX639345
Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345
Anti-Somatostatin receptor 3 antibody [HL2681] used in IHC (Paraffin sections) (IHC-P). GTX639345

Cat. No. GTX639345

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2681

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Somatostatin receptor 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Product Note

This antibody was raised against human Somatostatin receptor 3 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Somatostatin receptor 3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

somatostatin receptor 3 , SS-3-R , SS3-R , SS3R , SSR-28

Cellular Localization

Cell membrane

Background

This gene encodes a member of the somatostatin receptor protein family. Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This protein is functionally coupled to adenylyl cyclase. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345

GTX639345 WB Image

Comparison

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Somatostatin receptor 3 antibody [HL2681] (GTX639345) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345

GTX639345 WB Image

Comparison Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Somatostatin receptor 3 antibody [HL2681] (GTX639345) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345

GTX639345 WB Image

Human tissue extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 3 antibody [HL2681] (GTX639345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Somatostatin receptor 3 antibody [HL2681] used in IHC (Paraffin sections) (IHC-P). GTX639345

GTX639345 IHC-P Image

SSTR3 antibody detects SSTR3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
SSTR3 stained by SSTR3 antibody (GTX639345) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin receptor 3 antibody [HL2681] used in Western Blot (WB). GTX639345

GTX639345 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 3 antibody [HL2681] (GTX639345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Somatostatin receptor 3 antibody [HL2681] used in IHC (Paraffin sections) (IHC-P). GTX639345

GTX639345 IHC-P Image

Somatostatin receptor 3 antibody [HL2681] detects Somatostatin receptor 3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Somatostatin receptor 3 stained by Somatostatin receptor 3 antibody [HL2681] (GTX639345) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations