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Somatostatin receptor 3 antibody

Anti-Somatostatin receptor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130114
Anti-Somatostatin receptor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130114
Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114
Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

Cat No. GTX130114

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane extract , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human Somatostatin receptor 3.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886182

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

somatostatin receptor 3 , SS-3-R , SS3-R , SS3R , SSR-28

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR3 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in brain and pancreatic islets. SSTR3 is functionally coupled to adenylyl cyclase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Somatostatin receptor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130114

GTX130114 ICC/IF Image

Somatostatin receptor 3 antibody detects Somatostatin receptor 3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin receptor 3 stained by Somatostatin receptor 3 antibody (GTX130114) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Somatostatin receptor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130114

GTX130114 IHC-P Image

Somatostatin receptor 3 antibody detects Somatostatin receptor 3 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Somatostatin receptor 3 stained by Somatostatin receptor 3 antibody (GTX130114) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

GTX130114 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 3 antibody (GTX130114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

GTX130114 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SSTR3 antibody (GTX130114) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169