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Somatostatin receptor 3 antibody

Orthogonal
Anti-Somatostatin receptor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130114
Anti-Somatostatin receptor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130114
Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114
Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

Cat No. GTX130114

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Somatostatin receptor 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane extract , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human Somatostatin receptor 3.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

somatostatin receptor 3 , SS-3-R , SS3-R , SS3R , SSR-28

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR3 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in brain and pancreatic islets. SSTR3 is functionally coupled to adenylyl cyclase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Somatostatin receptor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130114

GTX130114 ICC/IF Image

Somatostatin receptor 3 antibody detects Somatostatin receptor 3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin receptor 3 stained by Somatostatin receptor 3 antibody (GTX130114) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Somatostatin receptor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130114

GTX130114 IHC-P Image

Somatostatin receptor 3 antibody detects Somatostatin receptor 3 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Somatostatin receptor 3 stained by Somatostatin receptor 3 antibody (GTX130114) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

GTX130114 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 3 antibody (GTX130114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Somatostatin receptor 3 antibody used in Western Blot (WB). GTX130114

GTX130114 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SSTR3 antibody (GTX130114) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169