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Sorbitol Dehydrogenase antibody [1D5]

Orthogonal Overexpression
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588
Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588

Cat. No. GTX83588

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D5

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Rat, Monkey
Package
100 μl ($519)
See all Sorbitol Dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10728939

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorbitol dehydrogenase , HEL-S-95n , SORD1

Background

Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.

Database

Research Area

DATA IMAGES

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83588

GTX83588 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Sorbitol Dehydrogenase plasmid using GTX83588 Sorbitol Dehydrogenase antibody [1D5].

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588

GTX83588 FACS Image

FACS analysis of HEK293T cells transfected with either Sorbitol Dehydrogenase plasmid(Red) or empty vector control plasmid(Blue) using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Dilution : 1:1000

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588

GTX83588 FACS Image

FACS analysis of Jurkat cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83588

GTX83588 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Flow cytometry (FACS). GTX83588

GTX83588 FACS Image

FACS analysis of HeLa cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588

GTX83588 WB Image

WB analysis of various cell lines using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Loading : 35 ug per lane
Dilution : 1:200

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588

GTX83588 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Loading : 5 ug per lane

Anti-Sorbitol Dehydrogenase antibody [1D5] used in Western Blot (WB). GTX83588

GTX83588 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099

REFERENCE

Application Reference

Fernando E Garc?a-Arroyo et al. Oxid Med Cell Longev 2020; 2020 : 8850266 Fluid Intake Restriction Concomitant to Sweetened Beverages Hydration Induce Kidney Damage.
Application : WB
Reactivity : Rat
Garc?a-Arroyo FE et al. Int J Mol Sci 2019; 20 (22) A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519