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Sorcin antibody [N1C3]

KO/KD
Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105
Anti-Sorcin antibody [N1C3] used in Immunoprecipitation (IP). GTX106105
Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105
Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105

Cat. No. GTX106105

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Sorcin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse heart , Molt-4 , HeLa

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sorcin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952006

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorcin , CP-22 , CP22 , SCN , V19

Cellular Localization

Cytoplasm

Background

Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.

Database

Research Area

DATA IMAGES

Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105

GTX106105 WB Image

KO/KD

Wild-type (WT) and Sorcin knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Sorcin antibody [N1C3] (GTX106105) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Sorcin antibody [N1C3] used in Immunoprecipitation (IP). GTX106105

GTX106105 IP Image

Sorcin antibody [N1C3] immunoprecipitates Sorcin protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Sorcin protein by 4 μg Sorcin antibody [N1C3] (GTX106105)
12 % SDS-PAGE
The immunoprecipitated Sorcin protein was detected by Sorcin antibody [N1C3] (GTX106105) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105

GTX106105 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX106105 diluted at 1:1000

Anti-Sorcin antibody [N1C3] used in Western Blot (WB). GTX106105

GTX106105 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
15% SDS PAGE
GTX106105 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169