Wild-type (WT) and Sorcin knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Sorcin antibody [N1C3] (GTX106105) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
15% SDS PAGE
GTX106105 diluted at 1:500

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX106105 diluted at 1:1000

Sorcin antibody [N1C3] immunoprecipitates Sorcin protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Sorcin protein by 4 μg Sorcin antibody [N1C3] (GTX106105)
12 % SDS-PAGE
The immunoprecipitated Sorcin protein was detected by Sorcin antibody [N1C3] (GTX106105) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]