APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Sortilin 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951976
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sortilin 1 , Gp95 , LDLCQ6 , NT3 , NTR3
Cellular Localization
Membrane; Single-pass type I membrane protein , Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential) , Endosome membrane; Single-pass type I membrane protein (Potential) , Golgi apparatus , Golgi stack membrane; Single-pass type
Background
This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857](/templates/default/js/lightbox/images/loading.gif "Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857")
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GTX110857 ICC/IF Image
Sortilin 1 antibody [C1C3] detects Sortilin 1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Sortilin 1 protein stained by Sortilin 1 antibody [C1C3] (GTX110857) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX110857 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX110857 diluted at 1:1000
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GTX110857 IHC-P Image
Sortilin 1 antibody [C1C3] detects Sortilin 1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Sortilin 1 antibody [C1C3] (GTX110857) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for Sortilin 1 antibody [C1C3] (GTX110857). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Sortilin 1 antibody [C1C3] (GTX110857). Be the first to share your experience with this product.
![Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857](/upload/website/prouct_img/small/GTX110857/GTX110857_40051_20170824_IFA_w_23060500_433.webp "Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857")
![Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857](/upload/website/prouct_img/small/GTX110857/GTX110857_40051_WB_M_brain_w_23060500_169.webp "Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857")
![Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857](/upload/website/prouct_img/small/GTX110857/GTX110857_40051_20151227_IHC-P_w_23060500_990.webp "Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857")
![Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857](/upload/website/prouct_img/normal/GTX110857/GTX110857_40051_20170824_IFA_w_23060500_433.webp "Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857")
![Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857](/upload/website/prouct_img/normal/GTX110857/GTX110857_40051_WB_M_brain_w_23060500_169.webp "Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857")
![Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857](/upload/website/prouct_img/normal/GTX110857/GTX110857_40051_20151227_IHC-P_w_23060500_990.webp "Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857")