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Sortilin 1 antibody [C1C3]

Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857
Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857
Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857

Cat No. GTX110857

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Mouse brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sortilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sortilin 1 , Gp95 , LDLCQ6 , NT3 , NTR3

Cellular Localization

Membrane; Single-pass type I membrane protein , Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential) , Endosome membrane; Single-pass type I membrane protein (Potential) , Golgi apparatus , Golgi stack membrane; Single-pass type

Background

This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sortilin 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110857

GTX110857 ICC/IF Image

Sortilin 1 antibody [C1C3] detects Sortilin 1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Sortilin 1 protein stained by Sortilin 1 antibody [C1C3] (GTX110857) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Sortilin 1 antibody [C1C3] used in Western Blot (WB). GTX110857

GTX110857 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX110857 diluted at 1:1000

Anti-Sortilin 1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110857

GTX110857 IHC-P Image

Sortilin 1 antibody [C1C3] detects Sortilin 1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Sortilin 1 antibody [C1C3] (GTX110857) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169