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Sortilin 1 antibody

Anti-Sortilin 1 antibody used in Western Blot (WB). GTX54854
Anti-Sortilin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54854
Anti-Sortilin 1 antibody used in Flow cytometry (FACS). GTX54854

Cat No. GTX54854

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat
Package
50 μl ($499)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.025% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 (Extracellular domain) of human Sortilin (Accession  : Q99523).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sortilin 1 , Gp95 , LDLCQ6 , NT3 , NTR3

Cellular Localization

Membrane; Single-pass type I membrane protein,Endoplasmic reticulum membrane,Endosome membrane,Lysosome membrane

Background

This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Sortilin 1 antibody used in Western Blot (WB). GTX54854

GTX54854 WB Image

WB analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates using GTX54854 Sortilin 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

Anti-Sortilin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54854

GTX54854 IHC-Fr Image

IHC-Fr analysis of rat neocortex tissue using GTX54854 Sortilin 1 antibody.
Panel A : Sortilin appears in cortical neurons (red).
Panel B : Staining of interneurons with mouse anti-parvalbumin (PV, green).
Panel C : Merge of Sortilin and PV demonstrates separate localization in neocortex.

Anti-Sortilin 1 antibody used in Flow cytometry (FACS). GTX54854

GTX54854 FACS Image

FACS analysis of THP-1 cells using GTX54854 Sortilin 1 antibody.
Black : Unstained cell
Red : Cell staining with isotype control antibody
Green : Cell staining with primary antibody
Antibody amount : 2.5 μg

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf