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Sortilin 1 antibody

KO/KD
Anti-Sortilin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54854
Anti-Sortilin 1 antibody used in Flow cytometry (FACS). GTX54854
Anti-Sortilin 1 antibody used in Western Blot (WB). GTX54854

Cat. No. GTX54854

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Sortilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 (Extracellular domain) of human Sortilin (Accession  : Q99523).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887894

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sortilin 1 , Gp95 , LDLCQ6 , NT3 , NTR3

Cellular Localization

Membrane; Single-pass type I membrane protein,Endoplasmic reticulum membrane,Endosome membrane,Lysosome membrane

Background

This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Sortilin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54854

GTX54854 IHC-Fr Image

IHC-Fr analysis of rat neocortex tissue using GTX54854 Sortilin 1 antibody.
Panel A : Sortilin appears in cortical neurons (red).
Panel B : Staining of interneurons with mouse anti-parvalbumin (PV, green).
Panel C : Merge of Sortilin and PV demonstrates separate localization in neocortex.

Anti-Sortilin 1 antibody used in Flow cytometry (FACS). GTX54854

GTX54854 FACS Image

FACS analysis of THP-1 cells using GTX54854 Sortilin 1 antibody.
Black : Unstained cell
Red : Cell staining with isotype control antibody
Green : Cell staining with primary antibody
Antibody amount : 2.5 μg

Anti-Sortilin 1 antibody used in Western Blot (WB). GTX54854

GTX54854 WB Image

WB analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates using GTX54854 Sortilin 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

REFERENCE

Application Reference

Robert P. Sparks et al. bioRxiv 2019; (Epub) Non-Canonical Binding of a Small Molecule to Sortilin Alters Cellular Trafficking of ApoB and PCSK9 in Liver Derived Cells.
Application : WB
Reactivity : Rat
Sparks RP et al. Biochem Biophys Res Commun 2016; 478 (2) : 546-52 sortilin facilitates vldl-b100 secretion by * sensitive mcardle rh7777 cells.
Application : WB
Reactivity : Rat
Sparks RP et al. Biochem Biophys Res Commun 2016; 479 (3) : 551-556 Phosphatidylinositol (3,4,5)-trisphosphate binds to sortilin and competes with neurotensin: Implications for very low density lipoprotein binding.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639