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Sox10 antibody

Anti-Sox10 antibody used in IHC (Whole mount) (IHC-Wm). GTX128374
Anti-Sox10 antibody used in IHC (Whole mount) (IHC-Wm). GTX128374

Cat. No. GTX128374

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Zebrafish, Chicken, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Sox10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885766

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY (sex determining region Y)-box 10 , cls/sox10 , zgc:100757

Database

Research Area

DATA IMAGES

Anti-Sox10 antibody used in IHC (Whole mount) (IHC-Wm). GTX128374

GTX128374 IHC-Wm Image

Sox10 antibody detects Sox10 protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Sox10 antibody (GTX128374) dilution: 1:100.

Anti-Sox10 antibody used in IHC (Whole mount) (IHC-Wm). GTX128374

GTX128374 IHC-Wm Image

sox10 antibody detects sox10 protein at neural crest cells and otic vesicle on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
sox10 antibody (GTX128374) dilution: 1:200.

REFERENCE

REVIEW

Sox10 antibody Cat. No. GTX128374

Rating ( Average 5 based on 4 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Nov-2021
Score :
Application Tested : IHC-Wm
Sample Species : Chk
Sample : Chicken Eighth-day embryo
Fixation : 4% PFA in 0.1M PBS, overnight, 4°C
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in 0.1M PBS, RT, 0.6Hr
Primary Antibody : 1:270, 4°C, 24Hr
Figure Note :
a. 10x image.
b-d. 40x images from a.
Red : Primary antibody
Blue : Hoechst staining of nuclear DNA
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29914980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Cells in 2019. PMID: 31450674
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Cells in 2019. PMID: 31450674
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169