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Sox3 antibody

KO/KD
Anti-Sox3 antibody used in IHC (Whole mount) (IHC-Wm). GTX132494
Anti-Sox3 antibody used in IHC (Whole mount) (IHC-Wm). GTX132494
Anti-Sox3 antibody used in Western Blot (WB). GTX132494
Anti-Sox3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132494

Cat. No. GTX132494

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm, ChIP assay

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Sox3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

whole zebrafish

Predict Reactivity

Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Sox3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY (sex determining region Y)-box 3 , id:ibd2036 , sb:cb493 , wu:fd02a08 , zgc:110279

Cellular Localization

Nucleus

Background

Transcriptional activator.

Database

Research Area

DATA IMAGES

Anti-Sox3 antibody used in IHC (Whole mount) (IHC-Wm). GTX132494

GTX132494 IHC-Wm Image

Sox3 antibody detects Sox3 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox3 antibody (GTX132494) dilution: 1:100.
Anti-Sox3 antibody used in IHC (Whole mount) (IHC-Wm). GTX132494

GTX132494 IHC-Wm Image

Sox3 antibody detects Sox3 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox3 antibody (GTX132494) dilution: 1:100.
Anti-Sox3 antibody used in Western Blot (WB). GTX132494

GTX132494 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Sox3 antibody (GTX132494) diluted at 1:1000.

Anti-Sox3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132494

GTX132494 IHC-Fr Image

The data was published in the journal Protein Cell in 2019. PMID: 30588557

REFERENCE

Application Reference

Ma L et al. Cell Death Dis 2024; 15 (3) : 229 METTL3-dependent m(6)A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.
Deshani C Ranawakage et al. Front Cell Dev Biol 2020; 8 : 598634 Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor.
Santos-Pereira JM et al. Nat Commun 2019; 10 (1) : 3049 Pioneer and repressive functions of p63 during zebrafish embryonic ectoderm specification.
Application : IHC-Wm, ChIP assay
Reactivity : Zebrafish
Ranawakage DC et al. Sci Rep 2019; 9 (1) : 6895 HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions.
Application : WB
Reactivity : Zebrafish
Hong Q et al. Protein Cell 2018; (Epub) Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish.
Application : WB
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169