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Sp7 antibody, C-term

Anti-Sp7 antibody, C-term used in Western Blot (WB). GTX50962
Anti-Sp7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX50962
Anti-Sp7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX50962
Anti-Sp7 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50962

Cat. No. GTX50962

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
50 μg ($409)
See all Sp7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human Sp7

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_626007

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp7 transcription factor , OI11 , OI12 , OSX , osterix

Cellular Localization

Nucleus

Background

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Sp7 antibody, C-term used in Western Blot (WB). GTX50962

GTX50962 WB Image

WB analysis of Jurkat cells using GTX50962 Sp7 antibody at 0.5μg/ml.

Anti-Sp7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX50962

GTX50962 IHC-P Image

IHC-P analysis of human intestine tissue using GTX50962 Sp7 antibody at 4.0-8.0μg/ml.

Anti-Sp7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX50962

GTX50962 IHC-P Image

The data was published in the journal Arthritis Res Ther in 2011. PMID: 21914169

Anti-Sp7 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50962

GTX50962 ICC/IF Image

The data was published in the journal BMC Dev Biol in 2015. PMID: 25879198

REFERENCE

Application Reference

Conserva E et al. Stem Cells Int 2019; 2019 : 4670560 Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells.
Application : ICC/IF
Reactivity : Human
Pisciotta A et al. BMC Dev Biol 2015; 15 : 14 Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations.
Application : ICC/IF
Reactivity : Human
Zavatti M et al. Life Sci 2013; 92 (20-21) : 993-1003 Ferutinin promotes proliferation and osteoblastic differentiation in human amniotic fluid and dental pulp stem cells.
Application : WB
Reactivity : Human
Kenzo Uchida et al. Arthritis Res Ther 2011; 13 (5) : R144 Ossification process involving the human thoracic ligamentum flavum: role of transcription factors.
Application : IHC-P
Reactivity : Human
Maraldi T et al. Tissue Eng Part A 2011; 17 (21-22) : 2833-43 Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409