GeneTex
  • Country / Location Selection

United States (US)

Spectrin beta 1 antibody [4C3]

Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808
Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808

Cat No. GTX22808

Host

Mouse

Clonality

Monoclonal

Clone Name

4C3

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100
ICC/IF 1:10-1:200
IHC-P 1:20-1:200
FACS 0.5-2 μg/test
IHC Assay dependent

Note :

ICC/IF
If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution.

IHC-P
If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution.

Not tested in other applications.

Calculated MW

246 kDa. ( Note )

Product Note

This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted in PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified human erythrocyte beta-1 spectrin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spectrin beta, erythrocytic , EL3 , HS2 , HSPTB1 , SPH2

Cellular Localization

Cytoplasm, cytoskeleton

Background

This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808

GTX22808 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808

GTX22808 ICC/IF Image

ICC/IF analysis of A431 cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808

GTX22808 ICC/IF Image

ICC/IF analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808

GTX22808 FACS Image

FACS analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808

GTX22808 WB Image

WB analysis of 25 ug of Hela cell lysates using GTX22808 Spectrin beta 1 antibody [4C3].
Dilution : 1:1000

Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808

GTX22808 IHC-P Image

IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20

Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808

GTX22808 FACS Image

FACS analysis of NIH/3T3 cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808

GTX22808 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20

Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808

GTX22808 FACS Image

FACS analysis of SH-SY5Y cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

REFERENCE

There are currently no references for Spectrin beta 1 antibody [4C3] (GTX22808). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Spectrin beta 1 antibody [4C3] (GTX22808). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf