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Spermine synthase antibody [N3C3]

Anti-Spermine synthase antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114783
Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783
Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783
Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783

Cat. No. GTX114783

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , Jurkat , rat kidney

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Spermine synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620063

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spermine synthase , MRSR , SPMSY , SRS , SpS

Background

The protein encoded by this gene belongs to the spermidine/spermine synthases family. This gene encodes an ubiquitous enzyme of polyamine metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Spermine synthase antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114783

GTX114783 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Spermine synthase(GTX114783) antibody at 1:200 dilution.

Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783

GTX114783 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
10% SDS PAGE
GTX114783 diluted at 1:1000

Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783

GTX114783 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Spermine synthase antibody [N3C3] (GTX114783) diluted at 1:500.

Anti-Spermine synthase antibody [N3C3] used in Western Blot (WB). GTX114783

GTX114783 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114783 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169