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Spinesin antibody [N3C3]

Anti-Spinesin antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX119497
Anti-Spinesin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119497
Anti-Spinesin antibody [N3C3] used in Western Blot (WB). GTX119497
Anti-Spinesin antibody [N3C3] used in Western Blot (WB). GTX119497

Cat. No. GTX119497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Spinesin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

MCF-7 , Mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Spinesin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane serine protease 5 , SPINESIN

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes a protein that belongs to the serine protease family. Serine proteases are known to be involved in many physiological and pathological processes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Spinesin antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX119497

GTX119497 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Spinesin(GTX119497) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Spinesin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119497

GTX119497 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Spinesin(GTX119497) antibody at 1:200 dilution.

Anti-Spinesin antibody [N3C3] used in Western Blot (WB). GTX119497

GTX119497 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX119497 diluted at 1:1000

Anti-Spinesin antibody [N3C3] used in Western Blot (WB). GTX119497

GTX119497 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX119497 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169