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Splicing Factor 1 (phospho Ser82) antibody

Anti-Splicing Factor 1 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX55309
Anti-Splicing Factor 1 (phospho Ser82) antibody used in Western Blot (WB). GTX55309

Cat. No. GTX55309

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Splicing Factor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from human Splicing Factor 1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 1 , BBP , D11S636 , MBBP , ZCCHC25 , ZFM1 , ZNF162

Cellular Localization

Nucleus

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-Splicing Factor 1 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX55309

GTX55309 IHC-P Image

IHC-P analysis of human brain tissue using GTX55309 Splicing Factor 1 (phospho Ser82) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Splicing Factor 1 (phospho Ser82) antibody used in Western Blot (WB). GTX55309

GTX55309 WB Image

WB analysis of extracts from HUVEC cells treated with anisomycin using GTX55309 Splicing Factor 1 (phospho Ser82) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399