GeneTex
United States (US)

Splicing factor 1 antibody [N1N3]

KO/KD
GTX104540_WB_19011506_201.jpg
GTX104540_40002_IFA_19011506_133.jpg

Cat No. GTX104540

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BBP antibody, D11S636 antibody, MBBP antibody, ZFM1 antibody, ZNF162 antibody, SF1 antibody, mammalian branch point-binding protein antibody, transcription factor ZFM1 antibody, zinc finger gene in MEN1 locus antibody, zinc finger protein 162 antibody, splicing factor 1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX104540_WB_19011506_201.jpg

GTX104540 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX104540 diluted at 1:1000

GTX104540_40002_IFA_19011506_133.jpg

GTX104540 ICC/IF Image

splicing factor 1 antibody [N1N3] detects splicing factor 1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] (GTX104540) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169