*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Knockdown/Knockout validation was supported by references (PMID:26990993)
Mouse, Rat, Zebrafish, Rabbit, Bovine, Xenopus laevis(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.77 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
splicing factor 1 , BBP , D11S636 , MBBP , ZCCHC25 , ZFM1 , ZNF162
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
GTX104540 WB Image
Sample(30 ug whole cell lysate)
7.5% SDS PAGE
GTX104540 diluted at 1:1000
GTX104540 ICC/IF Image
splicing factor 1 antibody [N1N3] detects splicing factor 1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] (GTX104540) diluted at 1:500.
Blue: Hoechst 33342 staining.