*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Knockdown/Knockout validation was supported by references (PMID:26990993)
Mouse, Rat, Zebrafish, Rabbit, Bovine, Xenopus laevis(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.77mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BBP antibody, D11S636 antibody, MBBP antibody, ZFM1 antibody, ZNF162 antibody, SF1 antibody, mammalian branch point-binding protein antibody, transcription factor ZFM1 antibody, zinc finger gene in MEN1 locus antibody, zinc finger protein 162 antibody, splicing factor 1 antibody
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
GTX104540 WB Image
Sample(30 ug whole cell lysate)
7.5% SDS PAGE
GTX104540 diluted at 1:1000
GTX104540 ICC/IF Image
splicing factor 1 antibody [N1N3] detects splicing factor 1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] (GTX104540) diluted at 1:500.
Blue: Hoechst 33342 staining.